D. melanogaster was the first organism to be presented in PhosphoPep,
and was obtained from the analysis Kc167 cells. Phosphopeptides were
enriched using 3 complementary methods, as described in
Nature Molecular Systems Biology .
The current Drosophila PhosphoPep contains over 10,000
high quality phosphorylation sites mapping to nearly 4,600
phosphoproteins (3,500 gene models) of D. melanogaster Kc167 cells and
thus being the most comprehensive phosphorylation map in any system to
Data on human phosphopeptides were obtained primarily from the publications of
Cell Signaling and the Gygi lab.
Overall the dataset consists of 3,980 phosphorylation sites.
C. elegans is a model organism whose development and genetics are very well characterized. Phosophopeptides were isolated from digested C. elegans whole organism lysates and analyzed using LTQ-FT and LTQ-Orbtitrap MS instruments.
Saccharomyces cerevisiae is a widely-used model organism whose popularity is enhanced with the release of the yeast deletion collection. This dataset contains nearly 9,000 identified phosphorylation sites, including over 5,800 with unambiguous phosphosite location. Most sites were identified from peptides which were separated according to their isoelectric point and analyzed using LTQ-FT and LTQ-Orbtitrap MS instruments.